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3Cs technology opens new horizons for production of highly complex CRISPR/Cas libraries.

06. Mar 2019

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As published today in eLife, a team around IBC2 Group Leader Manuel Kaulich has developed a new technology for generating CRISPR/Cas gene perturbation reagents. They dubbed their innovation as "3Cs", as gRNA are synthesized as covalently-closed circles. It enables the fast and, most importantly, cloning-free production of gRNA and shRNA libraries. By this, the 3Cs technology overcomes one of the major caveats of conventional library production. Another advantage is that the 3Cs technology uncouples sequence diversity from sequence distribution, thereby removing an often-observed bias in the production process and rendering high quality reagents in any order of magnitude. Groundbreaking discoveries can hardly ever be planned, and this one came over a coffee break. Manuel and Andreas Ernst, who at that time also headed an IBC2 group, were talking about their different areas of expertise, and suddenly the idea emerged on how to elegantly combine strategies used in phage display with gene editing. With support of Innovectis GmbH, Goethe University's Tech Transfer Office, they immediately applied for patent protection. To prove performance of 3Cs gene editing reagents in cells, together with the group of IBC2 Group Leader Anja Bremm a library targeting all human deubiquitinating enzymes (DUBs) was produced and their function for cell fitness identified. Then, the team around Manuel went on to generate the largest gRNA library known to date. This so-called oTGW (optimized truly genome wide) library targets 16.5 million unique sites throughout the human genome, both in coding and non-coding regions. The oTGW library has a great potential to fuel future functional genomics studies and is available for the scientific community via the Goethe University Depository. Together with IBC2 Director Ivan Dikic, Manuel set up the Frankfurt CRISPR/Cas Screening Center (FCSC).

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Christian Münch appointed as member of the Johanna Quandt Young Academy at Goethe (JQYA).

23. Jan 2019

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IBC2 group leader Christian Münch was recently admitted as a member to the JQ Young Academy. Within the "Science funding" program of JQYA, Christian will now receive financial support for his independent research group until October 2023. The Münch group is deciphering quality responses upon mitochondrial protein misfolding, a field with very high biomedical impact. Misfolding of mitochondrial proteins occurs e.g. as part of neurodegeneration, and little is known about how cellular response mechanisms contribute to the onset of respective diseases and their progression. The JQYA is an independent academy within Goethe University which foster researchers at the start of their independent careers.

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List of Highly Cited Researchers released.

27. Nov 2018

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IBC2 Director Ivan Dikic is one of the worlds' most influential scientists as revealed by Clarivate Analytics' Highly Cited Researchers List. He has been selected for his exceptional performance in the newly introduced category "Cross-Field", based on his multiple high impact papers published across several fields. The methodology used by Clarivate Analytics to curate this annual list aims at identifying scientists who have substantial contemporary impact. It is calculated from the number of highly cited papers produced, with a highly cited paper being defined as ranking amongst the top 1% as measured by citations for the field and year. Approximately 6,000 researchers worldwide are named in this years' list. The papers analyzed were published between 2006 to 2016. Besides Ivan Dikic, 12 other colleagues from Goethe University have been credited by the 2018 list. With this result, Goethe University is second best in Germany, only Heidelberg University scores higher. The global pole position is traditionally taken by Harvard University.

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Ivan Dikic is appointed as Max Planck Fellow.

01. Oct 2018

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As announced by the Max Planck Institute (MPI) of Biophysics today, IBC2 Director Ivan Dikic has been appointed as Fellow of the Max Planck Society and will start his 5-year-term immediately, including supervision of a small working group at the Frankfurt-based Institute. The Max Planck Fellow Programme has been set up to strengthen cooperation between outstanding university professors and Max Planck Society researchers. Research in the Dikic group is centered around two major cellular quality control pathways: the ubiquitin system and autophagy. As such they provide protection against various human diseases and are involved in almost all cellular signaling processes. The group covers a wide range of expertise to reveal structure-function relationships. Recently, the Dikic group revealed a novel ubiquitination mechanism induced by bacterial enzymes upon infection of human cells. In collaboration with colleagues at the MPI of Biophysics, the group now aims to resolve additional atomic details of this serine ubiquitination. A second area of mutual interest is in remodeling of endoplasmic reticulum via a process known as ER-phagy. Until now, very little is known about the mechanisms facilitating membrane targeting, bending and shaping during this selective form of autophagy. In addition, Ivan aims to also build strong links between colleagues at the MPI of Biophysics and the highly competitive cancer research programme at Goethe University. He is one of the founders of the Frankfurt Cancer Institute (FCI), which has recently received significant funding for building up a LOEWE center and a new research building.

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ERC Starting Grant for Christian Münch.

27. Jul 2018

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The European Research Council (ERC) awarded one of its prestigious Starting Grants to Emmy Noether group leader Christian Münch. The grant provides € 1.44 Mio to carry out a challenging project dubbed ‘mitoUPR’, in which Christian plans to unravel the impact of the mitochondrial unfolded protein response (UPRmt) on the cellular environment in mammalian cells. The UPRmt is a poorly understood mitochondrial stress response that activates upon protein misfolding in mitochondria. Münch’s interest in protein quality control processes dates back to his PhD at University of Cambridge (UK), when he was the first to describe the prion-like behavior of mutant SOD1 in ALS. During his postdoctoral tenure at Harvard University (Boston, USA), he discovered a new branch of the UPRmt controlling mitochondrial translation. Within his ERC project, Christian now plans to analyze how the UPRmt influences processes outside the mitochondrion. According to his central hypothesis, the regulation of cellular stress responses is centrally integrated, and he plans to unravel the role of UPRmt within those signaling networks. A second focus is on mitochondrial RNA granules, which are commonly associated with the organelle’s protein production machinery. Despite the fact that mutations in components of those granules are commonly associated with disease, so far little is known about their architecture and especially their regulation upon stress. To achieve his challenging aims, Christian relies on a range of technologies established at IBC2 and aims at developing an in vivo model for studying the UPRmt.

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Christian Münch joins SFB 1177 as project leader

11. Jul 2018

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Recently, the German Research Foundation (DFG) has approved Christian Münch’s supplementary proposal to study Mitophagy induction by the mitochondrial unfolded protein response within the framework of SFB 1177. Protein misfolding in mitochondria has been associated with numerous diseases, including neurodegeneration and cancer, and leads to activation of a mitochondrial stress response – the mitochondrial unfolded protein response (UPRmt). For the next two years, Christian Münch will address the role of mitophagy induction by the UPRmt to resolve mitochondrial protein folding defects.

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Wesch N, Kirkin V, Rogov V.V. 2020. Atg8-Family Proteins-Structural Features and Molecular Interactions in Autophagy and Beyond. Cells, 9:9, E2008

Wiechmann S, Maisonneuve P, Grebbin BM, Hoffmeister M, Kaulich M, Clevers H, Rajalingam K, Kurinov I, Farin HF, Sicheri F, Ernst A. 2020. Conformation-specific inhibitors of activated Ras GTPases reveal limited Ras dependency of patient-derived cancer organoids. J Biol Chem. pii: jbc.RA119.011025. 

Henkel V, Schürmanns L, Brunner M, Hamann A, Osiewacz HD. 2020. Role of sorting nexin PaATG24 in autophagy, aging and development of Podospora anserina. Mech Aging Dev, 186:111211

Kern A, Behl C. 2020. On-demand autophagic network adaptations upon limited lipid availability. Autophagy, 16:7, 1348-1350


Chaikuad A, Koschade SE, Stolz A, Zivkovic K, Pohl C, Shaid S, Ren H, Lambert LJ, Cosford NDP, Brandts CH & Knapp S. 2019. Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2). Biochem J, 476, 875-887.

Chakraborty D, Felzen V, Hiebel C, Stuerner E, Perumal N, Manicam C, Grus F, Wolfrum U, Behl C. 2019. Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress. Redox Biol, accepted

Christ MG, Huesmann H, Nagel H, Kern A & Behl C. 2019. Sigma-1 receptor activation induces autophagy and increases proteostasis capacity in vitro and in vivo. Cells, 8, pii: E211

Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, Fregno I, Raimondi A, Marazza A, Bruno G, Iavazzo M, Intartaglia D, Seczynska M, van Anken E, Conte I, De Matteis MA, Dikic I, Molinari M, Settembre C. 2019. A selective ER-phagy exerts procollagen quality control via a Calnexin- FAM134B complex. EMBO J, 38, pii:299847

Fottner M, Brunner AD, Bittl V, Horn-Ghetko, Jussupow A, Kaila VRI, Bremm A & Lang K. 2019. Sitespecific ubiquitylation and SUMOylation

Huber J, Obata M, Gruber J, Akutsu M, Löhr F, Rogova N, Guentert P, Dikic I, Kirkin V, Komatsu M, Dötsch V, Rogov VV. 2019. An atypical LIR motif within UFM1-activating enzyme 5 (UBA5) reveals an alternative function for GABARAP proteins. Autophagy, accepted.

Juretschke T, Beli P & Dikic I. 2019. Quantitative phosphoproteomics of selective autophagy receptors. pages 691–701, In: Autophagy; Methods in Molecular Biology. Vol. 1880 (eds. Ktistakis N and Florey O). Humana Press, New York.

Key J, Mueller AK, Gispert S, Matschke L, Wittig I, Corti O, Muench C, Decher N, Auburger G. 2019. Ubiquitylome profiling of Parkin-null brain reveals dysregulation of calcium homeostasis factors ATP1A2, Hippocalcin and GNA11, reflected by altered firing of noradrenergic neurons. Neurobiol Dis, 127, 114- 130.

Lim R, Sugino T, Nolte H, Andrade J, Zimmermann B, Shi C, Doddaballapur A, Ong YT, Wilhelm K, Fasse JWD, Ernst A, Kaulich M, Husnjak K, Boettger T, Guenther S, Braun T, Krueger M, Benedito R, Dikic I. Potente M. 2019. Deubiquitinase USP10 regulates Notch signaling in the endothelium. Science, 364, 188-193.

Nguyen TD, Shaid S, Vakhrusheva O, Koschade SE, Klann K, Thölken M, Baker F, Zhang J, Oellerich T, Sürün D, Derlet A, Haberbosch I, Eimer S, Osiewacz HD, Behrends C, Münch C, Dikic I, Brandts CH. 2019. Loss of the selective autophagy receptor p62 impairs murine myeloid leukemia progression and mitophagy. Blood, 133, 168-179.

Poluzzi C, Nastase MV, Zeng-Brouwers J, Roedig H, Hsieh LTH, Michaelis JB, Buhl EM, Rezende F, Manavski Y, Bleich A, Boor P, Brandes RP, Pfeilschifter J, Stelzer EHK, Muench C, Dikic I, Brandts C, Iozzo RV, Wygrecka M & Schaefer L. 2019. Biglycan evokes autophagy in macrophages via a novel CD44/Toll-like receptor 4 signaling axis: Impact on renal ischemia/reperfusion injury. Kidney Int, 95, 540- 562

Roedig H, Nastase MV, Wygrecka M & Schaefer L. 2019. Breaking down chronic inflammatory diseases: the role of biglycan in promoting a switch between inflammation and autophagy. FEBS J, doi: 10.1111/febs.14791

Van Well EM, Bader V, Patra M, Sánchez-Vicente A, Meschede J, Furthmann N, Schnack C, Blusch A, Longworth J, Petrasch-Parwez E, Mori K, Arzberger, Trümbach D, Angersbach L, Showkat C, Sehr DA, Berlemann LA, Goldmann P, Clement AM, Behl C, Woerner AC, Saft C, Wurst W, Haass C, Ellrichmann G, Gold R, Dittmar G, Hipp MS, Hartl FU, Tatzelt J, Winklhofer KF. 2019. A protein quality control pathway regulated by linear ubiquitination. EMBO J, pii: e100730.

Wegner M, Diehl V, Bittl V, de Bruyn R, Wiechmann S, Matthess Y, Hebel M, Hayes MG, Schaubeck S, Benner C, Heinz S, Bremm A, Dikic I, Ernst A, Kaulich M. 2019. Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome. eLife, 8, pii: e42549.


Di Rita A, Peschiaroli A, D Acunzo P, Strobbe D, Hu Z, Gruber J, Nygaard M, Lambrughi M, Melino G, Papaleo E, Dengjel J, El Alaoui S, Campanella M, Dötsch V, Rogov VV, Strappazzon F, Cecconi F. HUWE1 E3 ligase promotes PINK1/PARKIN-independent mitophagy by regulating AMBRA1 activation via IKKα. Nat Commun. 2018 Sep 14;9(1):3755. doi: 10.1038/s41467-018-05722-3. PubMed PMID: 30217973; PubMed Central PMCID: PMC6138665.

Kniss A, Kazemi S, Löhr F, Berger M, Rogov VV, Güntert P, Sommer T, Jarosch E, Dötsch V. Structural investigation of glycan recognition by the ERAD quality control lectin Yos9. J Biomol NMR. 2018 Jul 31. doi: 10.1007/s10858-018-0201-6. [Epub ahead of print] PubMed PMID: 30066206.

Meyer N, Zielke S, Michaelis JB, Linder B, Warnsmann V, Rakel S, Osiewacz HD, Fulda S, Mittelbronn M, Münch C, Behrends C, Kögel D. AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells. Autophagy. 2018;14(10):1693-1709. doi: 10.1080/15548627.2018.1476812. Epub 2018 Jul 21. PubMed PMID: 29938581; PubMed Central PMCID: PMC6135628.

Phelan JD, Young RM, Webster DE, Roulland S, Wright GW, Kasbekar M, Shaffer AL 3rd, Ceribelli M, Wang JQ, Schmitz R, Nakagawa M, Bachy E, Huang DW, Ji Y, Chen L, Yang Y, Zhao H, Yu X, Xu W, Palisoc MM, Valadez RR, Davies-Hill T, Wilson WH, Chan WC, Jaffe ES, Gascoyne RD, Campo E, Rosenwald A, Ott G, Delabie J, Rimsza LM, Rodriguez FJ, Estephan F, Holdhoff M, Kruhlak MJ, Hewitt SM, Thomas CJ, Pittaluga S, Oellerich T, Staudt LM. A multiprotein supercomplex controlling oncogenic signalling in lymphoma. Nature. 2018 Aug;560(7718):387-391. doi: 10.1038/s41586-018-0290-0. Epub 2018 Jun 20. PubMed PMID: 29925955.

Ziegler PK, Bollrath J, Pallangyo CK, Matsutani T, Canli Ö, De Oliveira T, Diamanti MA, Müller N, Gamrekelashvili J, Putoczki T, Horst D, Mankan AK, Öner MG, Müller S, Müller-Höcker J, Kirchner T, Slotta-Huspenina J, Taketo MM, Reinheckel T, Dröse S, Larner AC, Wels WS, Ernst M, Greten TF, Arkan MC, Korn T, Wirth D, Greten FR. Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. Cell. 2018 Jun 28;174(1):88-101.e16. doi: 10.1016/j.cell.2018.05.028. Epub 2018 Jun 14. PubMed PMID: 29909986.

Kern A, Spang N, Huesmann H, Behl C. Novel Modulators of Proteostasis: RNAi Screen of Chromosome I in a Heat Stress Paradigm in C. elegans. Cells. 2018 May 26;7(6). pii: E49. doi: 10.3390/cells7060049. PubMed PMID: 29861461; PubMed Central PMCID: PMC6025327.

Roedig H, Nastase MV, Frey H, Moreth K, Zeng-Brouwers J, Poluzzi C, Hsieh LT, Brandts C, Fulda S, Wygrecka M, Schaefer L. Biglycan is a new high-affinity ligand for CD14 in macrophages. Matrix Biol. 2018 May 17. pii: S0945-053X(18)30186-0. doi: 10.1016/j.matbio.2018.05.006. [Epub ahead of print] PubMed PMID: 29777767.

Harwardt MIE, Dietz MS, Heilemann M, Wohland T. SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules. Biophys J. 2018 May 22;114(10):2432-2443. doi: 10.1016/j.bpj.2018.03.013. Epub 2018 Apr 9. PubMed PMID: 29650369; PubMed Central PMCID: PMC6129459.

Dikic I, Elazar Z. Mechanism and medical implications of mammalian autophagy. Nat Rev Mol Cell Biol. 2018 Jun;19(6):349-364. doi: 10.1038/s41580-018-0003-4. Review. PubMed PMID: 29618831.

Kunz K, Piller T, Müller S. SUMO-specific proteases and isopeptidases of the SENP family at a glance. J Cell Sci. 2018 Mar 20;131(6). pii: jcs211904. doi: 10.1242/jcs.211904. Review. PubMed PMID: 29559551.

Fulda S. Targeting autophagy for the treatment of cancer. Biol Chem. 2018 Jun 27;399(7):673-677. doi: 10.1515/hsz-2018-0105. PubMed PMID: 29500917.

Heidelberger JB, Voigt A, Borisova ME, Petrosino G, Ruf S, Wagner SA, Beli P. Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function. EMBO Rep. 2018 Apr;19(4). pii: e44754. doi: 10.15252/embr.201744754. Epub 2018 Feb 21. PubMed PMID: 29467282; PubMed Central PMCID: PMC5891417.

Das CK, Linder B, Bonn F, Rothweiler F, Dikic I, Michaelis M, Cinatl J, Mandal M, Kögel D. BAG3 Overexpression and Cytoprotective Autophagy Mediate Apoptosis Resistance in Chemoresistant Breast Cancer Cells. Neoplasia. 2018 Mar;20(3):263-279. doi: 10.1016/j.neo.2018.01.001. Epub 2018 Feb 22. PubMed PMID: 29462756; PubMed Central PMCID: PMC5852393.

Kniss A, Schuetz D, Kazemi S, Pluska L, Spindler PE, Rogov VV, Husnjak K, Dikic I, Güntert P, Sommer T, Prisner TF, Dötsch V. Chain Assembly and Disassembly Processes Differently Affect the Conformational Space of Ubiquitin Chains. Structure. 2018 Feb 6;26(2):249-258.e4. doi: 10.1016/j.str.2017.12.011. Epub 2018 Jan 18. PubMed PMID: 29358025.

Carmona-Gutierrez D, Bauer MA, Zimmermann A, Aguilera A, Austriaco N, Ayscough K, Balzan R, Bar-Nun S, Barrientos A, Belenky P, Blondel M, Braun RJ, Breitenbach M, Burhans WC, Büttner S, Cavalieri D, Chang M, Cooper KF, Côrte-Real M, Costa V, Cullin C, Dawes I, Dengjel J, Dickman MB, Eisenberg T, Fahrenkrog B, Fasel N, Fröhlich KU, Gargouri A, Giannattasio S, Goffrini P, Gourlay CW, Grant CM, Greenwood MT, Guaragnella N, Heger T, Heinisch J, Herker E, Herrmann JM, Hofer S, Jiménez-Ruiz A, Jungwirth H, Kainz K, Kontoyiannis DP, Ludovico P, Manon S, Martegani E, Mazzoni C, Megeney LA, Meisinger C, Nielsen J, Nyström T, Osiewacz HD, Outeiro TF, Park HO, Pendl T, Petranovic D, Picot S, Polčic P, Powers T, Ramsdale M, Rinnerthaler M, Rockenfeller P, Ruckenstuhl C, Schaffrath R, Segovia M, Severin FF, Sharon A, Sigrist SJ, Sommer-Ruck C, Sousa MJ, Thevelein JM, Thevissen K, Titorenko V, Toledano MB, Tuite M, Vögtle FN, Westermann B, Winderickx J, Wissing S, Wölfl S, Zhang ZJ, Zhao RY, Zhou B, Galluzzi L, Kroemer G, Madeo F. Guidelines and recommendations on yeast cell death nomenclature. Microb Cell. 2018 Jan 1;5(1):4-31. doi: 10.15698/mic2018.01.607. Review. PubMed PMID: 29354647; PubMed Central PMCID: PMC5772036.

Frevert CW, Felgenhauer J, Wygrecka M, Nastase MV, Schaefer L. Danger-Associated Molecular Patterns Derived From the Extracellular Matrix Provide Temporal Control of Innate Immunity. J Histochem Cytochem. 2018 Apr;66(4):213-227. doi: 10.1369/0022155417740880. Epub 2018 Jan 1. PubMed PMID: 29290139; PubMed Central PMCID: PMC5958376.

Nastase MV, Janicova A, Roedig H, Hsieh LT, Wygrecka M, Schaefer L. Small Leucine-Rich Proteoglycans in Renal Inflammation: Two Sides of the Coin. J Histochem Cytochem. 2018 Apr;66(4):261-272. doi: 10.1369/0022155417738752. Epub 2018 Jan 1. PubMed PMID: 29290137; PubMed Central PMCID: PMC5958374.


Nastase MV, Zeng-Brouwers J, Wygrecka M, Schaefer L. Targeting renal fibrosis: Mechanisms and drug delivery systems. Adv Drug Deliv Rev. 2018 Apr;129:295-307. doi: 10.1016/j.addr.2017.12.019. Epub 2017 Dec 27. Review. PubMed PMID: 29288033.

Nastase MV, Zeng-Brouwers J, Beckmann J, Tredup C, Christen U, Radeke HH, Wygrecka M, Schaefer L. Biglycan, a novel trigger of Th1 and Th17 cell recruitment into the kidney. Matrix Biol. 2018 Aug;68-69:293-317. doi: 10.1016/j.matbio.2017.12.002. Epub 2017 Dec 15. PubMed PMID: 29253517.

Grumati P, Dikic I. Ubiquitin signaling and autophagy. J Biol Chem. 2018 Apr 13;293(15):5404-5413. doi: 10.1074/jbc.TM117.000117. Epub 2017 Nov 29. Review. PubMed PMID: 29187595; PubMed Central PMCID: PMC5900779.

Le Guerroué F, Eck F, Jung J, Starzetz T, Mittelbronn M, Kaulich M, Behrends C. Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Mol Cell. 2017 Nov 16;68(4):786-796.e6. doi: 10.1016/j.molcel.2017.10.029. PubMed PMID: 29149599.

Jung J, Behrends C. Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures. Bio Protoc. 2017 Sep 5;7(17):27982478. doi: 10.21769/BioProtoc.2540. PubMed PMID: 28966949; PubMed Central PMCID: PMC5619641.

Varga J, Greten FR. Cell plasticity in epithelial homeostasis and tumorigenesis. Nat Cell Biol. 2017 Oct;19(10):1133-1141. doi: 10.1038/ncb3611. Epub 2017 Sep 25. Review. PubMed PMID: 28945230.

Harwardt MIE, Young P, Bleymüller WM, Meyer T, Karathanasis C, Niemann HH, Heilemann M, Dietz MS. Membrane dynamics of resting and internalin B-bound MET receptor tyrosine kinase studied by single-molecule tracking. FEBS Open Bio. 2017 Aug 29;7(9):1422-1440. doi: 10.1002/2211-5463.12285. eCollection 2017 Sep. PubMed PMID: 28904870; PubMed Central PMCID: PMC5586345.

Henkel V, Warnsmann V, Osiewacz HD. Autophagic responses compensate mitochondrial impairments. Aging (Albany NY). 2017 Sep 12;9(9):1947-1948. doi: 10.18632/aging.101292. PubMed PMID: 28904241; PubMed Central PMCID: PMC5636663.

Aguilera-Gomez A, Zacharogianni M, van Oorschot MM, Genau H, Grond R, Veenendaal T, Sinsimer KS, Gavis EA, Behrends C, Rabouille C. Phospho-Rasputin Stabilization by Sec16 Is Required for Stress Granule Formation upon Amino Acid Starvation. Cell Rep. 2017 Aug 29;20(9):2277. doi: 10.1016/j.celrep.2017.08.054. PubMed PMID: 28854374.

Schwalm S, Beyer S, Frey H, Haceni R, Grammatikos G, Thomas D, Geisslinger G, Schaefer L, Huwiler A, Pfeilschifter J. Sphingosine Kinase-2 Deficiency Ameliorates Kidney Fibrosis by Up-Regulating Smad7 in a Mouse Model of Unilateral Ureteral Obstruction. Am J Pathol. 2017 Nov;187(11):2413-2429. doi: 10.1016/j.ajpath.2017.06.017. Epub 2017 Aug 12. PubMed PMID: 28807595.

Reilly R, Mroz MS, Dempsey E, Wynne K, Keely SJ, McKone EF, Hiebel C, Behl C, Coppinger JA. Targeting the PI3K/Akt/mTOR signalling pathway in Cystic Fibrosis. Sci Rep. 2017 Aug 9;7(1):7642. doi: 10.1038/s41598-017-06588-z. PubMed PMID: 28794469; PubMed Central PMCID: PMC5550428.

Wiechmann S, Gärtner A, Kniss A, Stengl A, Behrends C, Rogov VV, Rodriguez MS, Dötsch V, Müller S, Ernst A. Site-specific inhibition of the small ubiquitin-like modifier (SUMO)-conjugating enzyme Ubc9 selectively impairs SUMO chain formation. J Biol Chem. 2017 Sep 15;292(37):15340-15351. doi: 10.1074/jbc.M117.794255. Epub 2017 Aug 7. PubMed PMID: 28784659; PubMed Central PMCID: PMC5602394.

Polajnar M, Dietz MS, Heilemann M, Behrends C. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep. 2017 Sep;18(9):1572-1585. doi: 10.15252/embr.201643851. Epub 2017 Aug 6. PubMed PMID: 28784601; PubMed Central PMCID: PMC5579355.

Jung J, Behrends C. Multifaceted role of SMCR8 as autophagy regulator. Small GTPases. 2017 Jul 11:1-9. doi: 10.1080/21541248.2017.1346553. [Epub ahead of print] PubMed PMID: 28696821.

Warnsmann V, Meyer N, Hamann A, Kögel D, Osiewacz HD. A novel role of the mitochondrial permeability transition pore in (-)-gossypol-induced mitochondrial dysfunction. Mech Ageing Dev. 2018 Mar;170:45-58. doi: 10.1016/j.mad.2017.06.004. Epub 2017 Jul 3. PubMed PMID: 28684269.

Stürner E, Behl C. The Role of the Multifunctional BAG3 Protein in Cellular Protein Quality Control and in Disease. Front Mol Neurosci. 2017 Jun 21;10:177. doi: 10.3389/fnmol.2017.00177. eCollection 2017. Review. PubMed PMID: 28680391; PubMed Central PMCID: PMC5478690.

Rogov VV, Stolz A, Ravichandran AC, Rios-Szwed DO, Suzuki H, Kniss A, Löhr F, Wakatsuki S, Dötsch V, Dikic I, Dobson RC, McEwan DG. Structural and functional analysis of the GABARAP interaction motif (GIM). EMBO Rep. 2017 Aug;18(8):1382-1396. doi: 10.15252/embr.201643587. Epub 2017 Jun 27. PubMed PMID: 28655748; PubMed Central PMCID: PMC5538626.

Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MI, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I. Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy. Elife. 2017 Jun 15;6. pii: e25555. doi: 10.7554/eLife.25555. PubMed PMID: 28617241; PubMed Central PMCID: PMC5517149.

Galluzzi L, Baehrecke EH, Ballabio A, Boya P, Bravo-San Pedro JM, Cecconi F, Choi AM, Chu CT, Codogno P, Colombo MI, Cuervo AM, Debnath J, Deretic V, Dikic I, Eskelinen EL, Fimia GM, Fulda S, Gewirtz DA, Green DR, Hansen M, Harper JW, Jäättelä M, Johansen T, Juhasz G, Kimmelman AC, Kraft C, Ktistakis NT, Kumar S, Levine B, Lopez-Otin C, Madeo F, Martens S, Martinez J, Melendez A, Mizushima N, Münz C, Murphy LO, Penninger JM, Piacentini M, Reggiori F, Rubinsztein DC, Ryan KM, Santambrogio L, Scorrano L, Simon AK, Simon HU, Simonsen A, Tavernarakis N, Tooze SA, Yoshimori T, Yuan J, Yue Z, Zhong Q, Kroemer G. Molecular definitions of autophagy and related processes. EMBO J. 2017 Jul 3;36(13):1811-1836. doi: 10.15252/embj.201796697. Epub 2017 Jun 8. Review. PubMed PMID: 28596378; PubMed Central PMCID: PMC5494474.

Nastase MV, Janicova A, Wygrecka M, Schaefer L. Signaling at the Crossroads: Matrix-Derived Proteoglycan and Reactive Oxygen Species Signaling. Antioxid Redox Signal. 2017 Oct 20;27(12):855-873. doi: 10.1089/ars.2017.7165. Epub 2017 Jul 5. Review. PubMed PMID: 28510506.

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